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Chromatin Immunoprecipitation – A Method to Analyse Epigenet

by diagenodesa

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The field of biotechnology has increasingly become more wide spread with applications in diverse areas such as agriculture, environmental research and testing, molecular biology, genetic engineering, and clinical health care.


Advances in biotechnology contributed to the success of the Human Genome Project which uncovered major findings about the genome by identifying and mapping human genes. Knowledge from the HGP facilitated research in numerous areas of clinical interest such as epigenetics to gain a deeper understanding of disease at a molecular level for potential therapeutic procedures.

With implications in cancer and heritable diseases, the study of epigenetics and how genes are regulated has become crucial in cancer research for understanding predisposition factors and identifying biomarkers and potential drug targets. Of specific interest in the field is the interaction between proteins and DNA and genomic modifications, which give insight into how gene expression is regulated.


Epigenetic modifications and the location of DNA binding sites on the genome for a particular protein can be determined by chromatin immunoprecipitation (ChIP), a method developed by John T. Lis and David Gilmour in 1984. Various methodologies can be used to perform ChIP such ascarrier ChIP, fast ChIP, quick and quantitative ChIP, MicroChIP, and Matrix ChIP to name a few. Continual refinement of these methodologies has advanced ChIP to new levels.


Diagenode, a pioneer in the field of epigenetic marks and epigenome studies, offers state-of-the-art products that enable high reproducibility and consistency in epigenetics research. Diagenode is committed to epigenetics research, backed by a vast worldwide network of scientists and experts working in fields like cancer research, induced pluripotent stem cells (iPSC) or embryonic stem cells (ESC), and microRNA studies. The company offers a vast range of unique and robust products for studying epigenetic marks, unmatched by any competing alternatives. Products include antibodies, reagents, kits, and equipment for DNA or chromatin shearing, chromatin immunoprecipitation (ChIP, ChIP-Seq), methylated DNA immunoprecipitation (MeDIP, MeDIP-Seq), methyl binding domain (MethylCap, MBD), bisulfite conversion, automation ( IP-Star, SX8G Compact), and next generation sample preparation.


MeDIP  ||  5-hmC  || 5-mC

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